rat mrna & lncrna epitranscriptomic microarray  (Arraystar inc)

Journal: Frontiers in Molecular Biosciences

Article Title: Lipopolysaccharide Alters the m6A Epitranscriptomic Tagging of RNAs in Cardiac Tissue

doi: 10.3389/fmolb.2021.670160

Figure Lengend Snippet: M 6 A single-base site qPCR was used to confirm the microarray data for the top four methylated lncRNAs and top six methylated mRNAs in left ventricle tissue between the LPS and control groups. * p < 0.05 versus the Ctrl group. Ctrl, control; LPS, lipopolysaccharide.

Article Snippet: The cRNAs were combined and hybridized onto an Arraystar Rat mRNA&lncRNA Epitranscriptomic Microarray (4 × 44K, Arraystar) that contained 27,770 mRNA and 10,582 lncRNA degenerate probes.

Techniques: Microarray, Methylation, Control

Journal: Journal of Neuroinflammation

Article Title: The potential roles of m 6 A modification in regulating the inflammatory response in microglia

doi: 10.1186/s12974-021-02205-z

Figure Lengend Snippet: mRNA and lncRNA m 6 A modification profile changes in different primary rat microglia phenotypes. Hierarchical clustering of all samples revealed the non-random partitioning of samples into three major groups: M1-L vs M0-L; M2-L vs M0-L; and M2-L vs M1-L. Each column represents one sample and each row represents one mRNA ( A ) or lncRNA probe set ( B )

Article Snippet: The Cy3- and Cy5-labeled cRNA were mixed and hybridized to a rat m 6 A mRNA and lncRNA Epitranscriptomic Microarray (4 × 44 K, Arraystar) that contained 27,770 mRNA and 10,582 lncRNA.

Techniques: Modification

Journal: Journal of Neuroinflammation

Article Title: The potential roles of m 6 A modification in regulating the inflammatory response in microglia

doi: 10.1186/s12974-021-02205-z

Figure Lengend Snippet: Schematic overviews of the signaling pathways in which 10 m 6 A lncRNAs are probably involved. Ten m 6 A methylated lncRNAs, their regulated mRNAs and proteins are in the 14 upregulated signaling pathways. The red circles represent proteins, the green arrows represent the regulatory relationship of lncRNA to mRNA, the orange arrows show the mRNA and protein pair, and blue arrows indicate signaling pathways that proteins are involved

Article Snippet: The Cy3- and Cy5-labeled cRNA were mixed and hybridized to a rat m 6 A mRNA and lncRNA Epitranscriptomic Microarray (4 × 44 K, Arraystar) that contained 27,770 mRNA and 10,582 lncRNA.

Techniques: Protein-Protein interactions, Methylation